Acid Phosphatase 5, Tartrate Resistant (ACP5) BioAssay ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACP5 in rat serum, plasma, tissue homogenates and other biological fluids.Detection Range:0.78-50ng/mlSensitivity:0.32ng/mlIntra-Assay CV:<10% Inter-Assay CV:<12%Kit Components:*023084A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use*023084B: Standard, 2x1 vial023084C: Standard Diluent, 1x10ml or 1x20ml*023084D: Detection Reagent A (green), 1x60ul or 1x120ul*023084E: Detection Reagent B (red), 1x60ul or 1x120ul023084F: Assay Diluent A, 1x6ml or 1x12ml023084G: Assay Diluent B, 1x6ml or 1x12ml023084H: TMB Substrate, 1x4.5ml or 1x9ml023084K: Stop Solution, 1x3ml or 1x6ml023084L: Wash Buffer, 30X, 1x10ml or 1x20mlPrecaution:The Stop Solution (023084K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *023084A, *023084B, *023084D and *023084E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.