Alanine Aminotransferase (ALT) BioAssay ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ALT in rat serum, plasma, tissue homogenates and other biological fluids.Detection Range: 0.78 -50ng/mlSensitivity: 0.31ng/mlIntra-Assay: CV<10%Inter-Assay: CV<12%Kit Components:*023207A: Microtiter Plate, 1x96wells Pre-coated, ready to use*023207B: Standard, 50ng, 2x1vial 023207C: Standard Diluent, 1×20ml*023207D: Detection Reagent A (green) 1×120ul *023207E: Detection Reagent B (red) 1×120ul 023207F: Assay Diluent A, 2x 1×6ml023207G: Assay Diluent B, 2× 1×6ml023207H: TMB Substrate 1×9ml023207J: Stop Solution 1×6ml023207K: Wash Buffer, 30X 1×20ml Precaution:023207J: Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *023207A, *023207B, *023207D Stock and *023207E Stock at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.