Apolipoprotein B (APOB) BioAssay ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of APOB in rat serum, plasma and other biological fluids.Detection Range:6.25-400ng/mlSensitivity:2.88ng/mlIntra-Assay CV:<10% Inter-Assay CV:<12%Kit Components:*023505A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*023505B: Standard, 2x1 vial023505C: Standard Diluent, 1x20ml*023505D: Detection Reagent A (green), 1x120ul*023505E: Detection Reagent B (red), 1x120ul023505F: Assay Diluent A, 1x12ml023505G: Assay Diluent B, 1x12ml023505H: TMB Substrate, 1x9ml023505K: Stop Solution, 1x6ml023505L: Wash Buffer, 30X, 1x20mlPrecaution:The Stop Solution (023505K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *023505A, *023505B, *023505D and *023505E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
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保存建议
建议收到Apolipoprotein B (APOB) BioAssay ELISA Kit (Rat)产品后将其置于4°C/-20°C保存。