Glial Fibrillary Acidic Protein (GFAP) BioAssay ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GFAP in rat serum, plasma and other biological fluids.Detection Range:15.62-1000pg/mlSensitivity:6.3pg/mlIntra-Assay CV:<10%Inter-Assay CV:<12%Kit Components:*025284A: Microtiter Strips, 1x48 wells, Pre-coated, ready to use*025284B: Standard, 2x1vial025284C: Standard Diluent, 1x10ml 025284D: Detection Reagent A (green), 1x60ul *025284E: Detection Reagent B (red), 1x60ul025284F: Assay Diluent A, 1x6ml 025284G: Assay Diluent B, 1x6ml 025284H: TMB Substrate, 1x4.5ml 025284K: Stop Solution, 1x3ml 025284L: Wash Buffer, 30X, 1x10mlPrecaution:The Stop Solution (025284K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *025284A, *025284B, *025284D and *025284E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
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保存建议
建议收到Glial Fibrillary Acidic Protein (GFAP) BioAssay ELISA Kit (Rat)产品后将其置于4°C/-20°C保存。