Hepatocyte Growth Factor (HGF) BioAssay ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of HGF in rat serum, plasma, tissue homogenates, cell lysates and other biological fluids.Detection Range:15.6-1000pg/mlSensitivity:6.2pg/mlIntra-Assay CV:<10% Inter-Assay CV:<12%Kit Components:*025638A: Microtiter Strips, 1x96 wells*025638B: Standard, 2x1 vial025638C: Standard Diluent, 1x20ml*025638D: Detection Reagent A (green), 1x120ul*025638E: Detection Reagent B (red), 1x120ul025638F: Assay Diluent A, 1x12ml025638G: Assay Diluent B, 1x12ml025638H: TMB Substrate, 1x9ml*025638J: Positive Control, 82.4pg/ml, 1x1 vial025638K: Stop Solution, 1x6ml025638L: Wash Buffer, 30X, 1x20mlPrecaution:The Stop Solution (025638K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *025638A, *025638B, *025638D, *025638E and *025638J at -20°C. After reconstitution of *025638J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.