Interleukin 1 Beta (IL1b) BioAssay ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL1b in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Detection Range:6.25-400pg/mlSensitivity:2.47pg/mlntra-Assay CV:<10% Inter-Assay CV:<12%Kit Components:*026023A: Microtiter Strips, 1x96 wells, Pre-coated; ready to use*026023B: Standard, 2x1vial 026023C: Standard Diluent, 1x20ml*026023D: Detection Reagent A, 1x120ul *026023E: Detection Reagent B, 1x120ul 026023F: Assay Diluent A, 1x12ml026023G: Assay Diluent B, 1x12ml026023H: TMB Substrate, 1x9ml026023K: Stop Solution, 1x6ml026023L: Wash Buffer, 30X, 1x20ml Precaution:The Stop Solution (026023K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *026023A, *026023B, *026023D and *026023E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.