Islet Amyloid Polypeptide (IAPP) BioAssay ELISA Kit (Rat) is a competitive inhibition immunoassay for in vitro quantitative measurement of IAPP in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Detection Range:6.17-500pg/mlSensitivity:2.78pg/mlIntra-Assay CV: <10%Inter-Assay CV: <12%Kit Components:*026301A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*026301B: Standard, 2x1 vial026301C: Standard Diluent, 1x20ml*026301D: Detection Reagent A (green), 1x1vial*026301E: Detection Reagent B (red), 1x120ul026301F: Assay Diluent A, 1x12ml026301G: Assay Diluent B, 1x12ml026301H: TMB Substrate, 1x9ml026301K: Stop Solution, 1x6ml026301L: Wash Buffer, 30X, 1x20ml026301M: Detection Reagent A Diluent, 1x300ulPrecaution:The Stop Solution (026301K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *026301A, *026301B, *026301D and *026301E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.5. Aspirate and wash 5 times.6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.7. Add 50ul Stop Solution. Read at 450nm immediately.