Monocyte Chemotactic Protein 1 (MCP1) BioAssay ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of MCP1 in rat serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.Detection Range:0.156-10ng/mlSensitivity:0.061ng/mlIntra-Assay: CV < 10%Inter-Assay: CV < 12%Kit Components:*026853A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use*026853B: Standard, 2x1 vial 026853C: Standard Diluent, 1x20ml*026853D: Detection Reagent A (100X), 1x120ul *026853E: Detection Reagent B (100X), 1x120ul 026853F: Assay Diluent A (2X), 1x6ml026853G: Assay Diluent B (2X), 1x6ml026853H: TMB Substrate, 1x9ml*026853J: Positive Control, 99.3pg/ml, 1x1 vial026853K: Stop Solution, 1x6ml026853L: Wash Buffer, 30X, 1x20mlPrecaution:The Stop Solution (026853K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *026853A, *026853B, *026853D, *026853E and *026853J at -20°C. After reconstitution of *026853J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Add 100ul working Detection Reagent A immediately. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul working Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.
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保存建议
建议收到Monocyte Chemotactic Protein 1 (MCP1) BioAssay ELISA Kit (Rat)产品后将其置于4°C/-20°C保存。