Noggin (NOG) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of NOG in human serum, plasma, tissue homogenates and other biological fluids. Species homology: Mouse, ratDetection Range:0.156-10ng/mlSensitivity:0.063ng/mlIntra-Assay CV:< 10% Inter-Assay CV:< 12%Kit Components:*027177A: Microtiter Strips, 1x48 wells or1x96 wells, Pre-coated, ready to use*027177B: Standard, 2x1 vial027177C: Standard Diluent, 1x10ml or 1x20ml*027177D: Detection Reagent A (green), 1x60ul or 1x120ul*027177E: Detection Reagent B (red), 1x60ul or 1x120ul027177F: Assay Diluent A, 1x6ml or 1x12ml027177G: Assay Diluent B, 1x6ml or 1x12ml027177H: TMB Substrate, 1x4.5ml or 1x9ml*027177J: Positive Control, 2.15ng/ml, 1x1vial 027177K: Stop Solution, 1x3ml or 1x6ml027177L: Wash Buffer, 30X, 1x10ml or 1x20mlPrecaution:The Stop Solution (027177K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *027177A, *027177B, *027177D, *027177E and *027177J at -20°C. After reconstitution of *027177J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards;2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.