FGF-23, Rat, BioAssay ELISA Kit (Fibroblast Growth Factor 23, Fgf23)-FGF-23, Rat, BioAssay ELISA Kit (Fibroblast Growth Factor 23, Fgf23)代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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细胞分析试剂盒

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中文名称: FGF-23, Rat, BioAssay ELISA Kit (Fibroblast Growth Factor 23, Fgf23)

英文名字: FGF-23, Rat, BioAssay ELISA Kit (Fibroblast Growth Factor 23, Fgf23)

供应商: USBiological

产品货号: 029475

产品报价: ￥1/96Tests

产品说明书

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: FGF-23, a phosphate-regulating hormone, plays a critical role in phosphorus homeostasis. The study of FGF-23 is important in understanding the role it plays in such processes as bone remodeling and kidney function. FGF-23 is responsible for the inhibition of renal tubular reabsorption of inorganic phosphorus and, consequently, the regulation of phosphorus homeostasis. FGF-23 mRNA is expressed in the brain, in the thymus at low levels, in dendritic cells, with the highest expression in osteocytes and osteoblasts during phases of active bone remodeling. Because circulating levels of FGF-23 are regulated by vitamin D and phosphorus, normal skeletal and soft tissue development require vitamin D and a functional vitamin D signaling pathway. Diseases resulting from aberrant FGF-23 expression include inherited forms of rickets, certain kidney diseases and phosphorus-related tumorsIntended Use: FGF-23, Rat, BioAssay ELISA Kit is used for the non-radioactive quantification of FGF-23 in rat serum, plasma, and tissue culture samples. This kit specifically measures native rat FGF-23. One kit is sufficient to measure 41 unknown samples in duplicate. This kit is for research use only.Detection Range:0.82-20ng/mlSensitivity:0.05ng/mlPrecision:Intra-Assay %CV: <10Inter-Assay %CV: <10Specificity:The antibody pair used in this assay is specific to rat FGF-23 and does not significantly crossreact to the following molecules/hormones tested: other FGFsPrinciple of the Test:This assay is a Sandwich ELISA based, sequentially, on: 1) concurrent capture of rat FGF-23 molecules from samples to the wells of a microtiter plate coated with a polyclonal rabbit anti-FGF-23 antibody, and binding of a second biotinylated polyclonal goat anti-FGF-23 antibody to the captured molecules, 2) washing of unbound materials from samples, 3) binding of streptavidin-horseradish peroxidase conjugate to the immobilized biotinylated antibodies, 4) washing of excess of free enzyme conjugates, and 5) quantification of immobilized antibody-enzyme conjugates by monitoring horseradish peroxidase activities in the presence of the substrate 3,3’,5,5’-tetramethylbenzidine. The enzyme activity is measured spectrophotometrically by the increased absorbance at 450–590nm after acidification of formed products. Since the increase in absorbance is directly proportional to the amount of captured rat FGF-23 in the unknown sample, the latter can be derived by interpolation from a reference curve generated in the same assay with reference standards of known concentrations of rat FGF-23.Kit Components:029475A: FGF-23 ELISA Microtiter Strips, 1x96 wells029475B: HRP Wash Buffer Concentrate, 10X, 2x50ml029475C: FGF-23 Standard, 1x1 vial029475D: FGF-23 Quality Control 1, 0.22-0.46ng/ml, 1x1 vial029475E: FGF-23 Quality Control 2, 1.9-4.0ng/ml, 1x1 vial029475F: Matrix Solution, 1x1.5ml029475G: Assay Buffer, 1x12ml029475H: FGF-23 Detection Antibody, 1x12ml029475J: Streptavidin (HRP), 1x12ml029475K: TMB Substrate, 1x12ml029475L: Stop Solution, 1x12mlStorage and Stability:Recommended storage for kit components is 4°C. Stable for 6 months from the date of receipt. All components are shipped and stored at 4°C. Reconstituted standards and controls can be frozen for future use, but repeated freeze thaws should be avoided. Do not mix reagents from different kits.

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保存建议: 建议收到FGF-23, Rat, BioAssay ELISA Kit (Fibroblast Growth Factor 23, Fgf23)产品后将其置于4°C/-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。