Ca²+-dependent cysteine proteinase with low Ca²ᶧrequirement (half maximal activation = 2 uM). Participates in the ATP release reaction of platelets stimulated with thrombin. Involves in apoptosis activation.
序列
人Calpain I蛋白,有活性的蛋白产品,又称Calcium-activated neutral proteinase 2, Short name=CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, Short name=M-calpain.其对应NCBI基因号为181186,NCBI基因别称为Granzyme B;该基因种属来源为人源,对应Accession号为AAA75490.该蛋白产品又称Active Human Calpain I, Human CAPN1, Human Calpain-1, Calpain-1, active calpain等.
来源宿主
提供的【Calpain I】人Calpain I蛋白,有活性的产品的来源为人红细胞中提取纯化.
溶解建议
提供的人Calpain I蛋白,有活性的 产品为液态形式以1 mg/ml浓度溶解于20 mM 咪唑-HCl, 5 mM β-巯基乙醇, 1 mM EDTA, 1 mM EGTA和30%甘油, pH 6.8溶液中.