PDE4A, BioAssay Kit (cAMP-specific 3',5'-cyclic Phosphodiesterase 4A, DPDE2, PDE46, DPDE2)-PDE4A, BioAssay Kit (cAMP-specific 3',5'-cyclic Phosphodiesterase 4A, DPDE2, PDE46, DPDE2)代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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中文名称: PDE4A, BioAssay Kit (cAMP-specific 3',5'-cyclic Phosphodiesterase 4A, DPDE2, PDE46, DPDE2)

英文名字: PDE4A, BioAssay Kit (cAMP-specific 3',5'-cyclic Phosphodiesterase 4A, DPDE2, PDE46, DPDE2)

供应商: USBiological

产品货号: 137340

产品报价: ￥1/96Tests

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4A is widely expressed in brain tumors and promotes their growth and its inhibitor Rolipram is identified as a potent anti- inflammatory agent. The PDE4A Assay Kit is designed for identification of PDE4A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4A to the binding agent.Intended Use:Designed for identification of PDE4A inhibitors using fluorescence polarization.Test Principle:The PDE4A inhibitor screening assay kit comes in a convenient 96-well format, including purified PDE4A1 enzyme, fluorescently labeled PDE4A substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE4A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.Kit Components:PDE4A1a: 1x300ngFAM-Cyclic-3’, 5’-AMP: 20uM: 1x50ulPDE assay buffer: 1x12mlBinding Agent: 1x100ulBinding Agent Diluent: 1x10mlBlack, low binding NUNC black microtiter plate: 1 plateStorage and Stability:Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

产品特点: 针对PDE4A, BioAssay Kit (cAMP-specific 3',5'-cyclic Phosphodiesterase 4A, DPDE2, PDE46, DPDE2)该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到PDE4A, BioAssay Kit (cAMP-specific 3',5'-cyclic Phosphodiesterase 4A, DPDE2, PDE46, DPDE2)产品后将其置于-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。