Sandwich ELISA quantitative detection kit for Rat CCL4/MIP-1beta in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1β, one or more additional β chemokine receptors that transduce MIP-1β signals must also exist. MIP-1α and MIP-1β have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many α as well as β chemokines (11, 14).Assay Range:15.6pg/ml-1000pg/mlSensitivity:< 10 pg/mlCrossreactivity:There is no detectable cross-reactivity with other relevant proteins.Uniprot ID:P50230Entrez Gene ID:116637Kit Components:96-well plate pre-coated with anti-CCL4/MIP-1 beta, RatLyophilized recombinant CCL4/MIP-1 beta, Rat standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10mlStorage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.