Sandwich ELISA quantitative detection kit for Human Granzyme B in cell culture supernates, serum and plasma (heparin, EDTA).Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.Assay Range:15.6pg/ml-1000pg/mlSensitivity:< 10 pg/mlCrossreactivity:There is no detectable cross-reactivity with other relevant proteins.Uniprot ID:P10144Entrez Gene ID:3002Kit Components:96-well plate pre-coated with anti-Granzyme B, HumanLyophilized recombinant Granzyme B, Human standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10mlStorage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
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保存建议
建议收到Granzyme B BioAssay ELISA Kit, Human产品后将其置于4°C/-20°C保存。