Sandwich ELISA quantitative detection kit for Rat IL-18 in cell culture supernates, serum and plasma (heparin, EDTA).Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3 Assay Range:15.6pg/ml-1000pg/mlSensitivity:< 1 pg/mlCrossreactivity:There is no detectable cross-reactivity with other relevant proteins.Uniprot ID:P97636Entrez Gene ID:29197Kit Components:96-well plate pre-coated with anti-IL-18, RatLyophilized recombinant IL-18, Rat standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10mlStorage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.