BAFF (B cell activating factor), also known as BlyS (B lymphocyte stimulator) and TALL-1 (TNF- and APOL-related leukocyte expressed ligand 1) is a member of the TNF superfamily designated TNFSF13B. BAFF is expressed by monocytes and dendritic cells in the form of a membrane-anchored homotrimer which may be shed from the cell surface by Furin. BAFF binds BAFF R/TNFRSF13C, BCMA/TNFRSF17 and TACI/TNFRSF13B receptors to regulate B cell homeostasis.Sample Type:Cell culture supernates, serum, EDTA plasma, and heparin plasma.Sensitivity:The mean MDD was 0.56pg/ml. The Minimum Detectable Dose (MDD) was determined by adding two standard deviations to the MFI of twenty zero standard replicates and calculating the corresponding concentration. Range:~0.19-1.06pg/mlSpecificity:Recognizes natural and recombinant human BAFF/BLyS. Test Principle:Human BAFF/BLyS/TNFSF13B BioAssay Kit afford the user the benefit of multianalyte analysis of biomarkers in a complex sample. For each sample type, a single, multipurpose diluent is used to optimize recovery, linearity, and reproducibility. Such a multipurpose diluent may not optimize any single analyte to the same degree that a unique diluent selected for analysis of that analyte can optimize conditions. Therefore, some performance characteristics may be more variable than those for assays designed specifically for single analyte analysis.Kit Components:Microparticle Concentrate, 100X concentrated, 1x0.075mlBiotin-Antibody Concentrate, 100X concentrated, 1x0.075mlStorage and Stability:Store powder at 4°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.