Mucin 13, Cell Surface Associated (MUC13) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of MUC13 in human tissue homogenates, cell lysates and other biological fluids.Detection Range:0.156-10ng/mlSensitivity:0.055ng/mlIntra-Assay CV: <10%Inter-Assay CV: <12%Kit Components:*152832A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*152832B: Standard, 2x1 vial152832C: Standard Diluent, 1x20ml*152832D: Detection Reagent A (green), 1x120ul*152832E: Detection Reagent B (red), 1x120ul152832F: Assay Diluent A, 1x12ml152832G: Assay Diluent B, 1x12ml152832H: TMB Substrate, 1x9ml152832K: Stop Solution, 1x6ml152832L: Wash Buffer, 30X, 1x20mlPrecaution:The Stop Solution (152832K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *152832A, *152832B, *152832D and *152832E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.