Pancreatic Polypeptide (PP) BioAssay ELISA Ki is a competitive inhibition immunoassay for in vitro quantitative measurement of cortisol in rat serum, plasma and other biological fluids.Detection Range:24.69-2000pg/ml Sensitivity:8.85pg/mlIntra-Assay CV:<10% Inter-Assay CV:<12%Kit Components:*152921A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use*152921B: Standard, 2x1 vial152921C: Standard Diluent, 1x10ml or 1x20ml*152921D: Detection Reagent A (green), 1x60ul or 1x120ull*152921E: Detection Reagent B (red), 1x60ul or 1x120ul 152921F: Assay Diluent A, 1x6ml or 1x12ml152921G: Assay Diluent B, 1x6ml or 1x12ml152921H: TMB Substrate, 1x4.5ml or 1x9ml152921K: Stop Solution,1x3ml or 1x6ml152921L: Wash Buffer, 30X, 1x10ml or 1x20mlPrecaution:The Stop Solution (152921K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *15292A, *15292B, *15292D and *15292E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.5. Aspirate and wash 5 times.6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.7. Add 50ul Stop Solution. Read at 450nm immediately.