Coelenterazine is a luciferin, a light-emitting biomolecule that serves as a substrate for luciferases or as a constituent of photoproteins.{22804} The oxidation of coelenterazine to coelenteramide is accompanied by emission of blue light (emission maximum = 460-470 nm). Luciferase-mediated oxidation of coelenterazine or a derivative is used as an energy donor, typically to a form of green fluorescent protein (GFP), in bioluminescent resonance energy transfer (BRET) studies.{26295,26296,26290} Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that produces an emission peak near 400 nm following conversion by Renilla luciferase (Rluc).{26297,26291} It is used in BRET2 protocols, whereas coelenterazine h, which has a 475 nm Rluc emission peak, is used in BRET1 protocols.{26296} The emission peak of coelenterazine 400a is commonly paired with class 1 and 3 GFPs, including GFP2 and GFP10.{26296,26291} BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.{26297,26291,26294}
产品描述
A bisdeoxy derivative of coelenterazine that produces an emission peak near 400 nm following conversion by Renilla luciferase; commonly used in BRET2 protocols to evaluate protein-protein interactions