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中文名称: C-Peptide BioAssay ELISA Kit (Human, Mouse, Rat)

英文名字: C-Peptide BioAssay ELISA Kit (Human, Mouse, Rat)

供应商: USBiological

产品货号: 167774

产品报价: ￥1/100Tests

产品说明书

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产品新闻

背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: C-Peptide BioAssay ELISA Kit (Human, Mouse, Rat) is used for quantitative protein detection, establishing normal range and validation of antibody array results.Sample Type:Serum, plasma and cell culture media etc.Specificity:The capture antibody provided in this kit recognizes human, mouse and rat C-Peptide. EIA-CPE-1 only detects C-peptide, not Insulin A or B chain.Sensitivity:The intra-Assay reproducibility is CV<10% and inter-Assay is CV<12%. Range:The detection range for the kit is 0.1-1000ng/mlTest Principle:C-Peptide Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting C-Peptide peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- C-Peptide antibody, both biotinylated C-Peptide peptide and peptide standard or targeted peptide in samples interacts competitively with the C-Peptide antibody. Uncompeted (bound) biotinylated C-Peptide peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of C-Peptide peptide in the standard or samples. This is due to the competitive binding to C-Peptide antibody between biotinylated C-Peptide peptide and peptides in standard or samples. A standard curve of known concentration of C-Peptide peptide can be established and the concentration of C-Peptide peptide in the samples can be calculated accordingly. The minimum detectable concentration of C-Peptide is 772pg/ml. Kit Components:C-Peptide Microplate (Item A), 1x96wellsWash Buffer Concentrate (Item B), 1x25mlStandard C-Peptide peptide (Item C), 2x vialsAssay Diluent A (Item D), 1x30mlAssay Diluent B (Item E), 1x15 mlBiotinylated C-Peptide peptide (Item F), 2x vialsHRP-Streptavidin concentrate (Item G), 1x600ulTMB One-Step Substrate Reagent (Item H), 1x12mlStop Solution (Item I), 1x8mlPositive control (Item M), 1x vialAnti- C-Peptide polyclonal antibody (Item N), 2x vialsStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

产品特点: 针对C-Peptide BioAssay ELISA Kit (Human, Mouse, Rat)该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到C-Peptide BioAssay ELISA Kit (Human, Mouse, Rat)产品后将其置于-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。