Obestatin BioAssay ELISA Kitis an in vitro quantitative assay for detecting Obestatin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Obestatin antibody, both biotinylated Obestatin peptide and peptide standard or targeted peptide in samples interacts competitively with the Obestatin antibody. Uncompeted (bound) biotinylated Obestatin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Obestatin peptide in the standard or samples. This is due to the competitive binding to Obestatin antibody between biotinylated Obestatin peptide and peptides in standard or samples. A standard curve of known concentration of Obestatin peptide can be established and the concentration of Obestatin peptide in the samples can be calculated accordingly. Sample Type:Serum, plasma and cell culture mediumSpecificity:The capture antibody provided in this kit recognizes human, mouse and rat Obestatin. This EIA kit shows no cross-reactivity with Ghrelin, Nesfatin, Angiotensin II, NPY and APC.Sensitivity:Intra-assay: CV<10%Inter-assay: CV<12%. Range:0.1-1000pg/ml. Kit Components:167927A: Microtiter Strips, 1x96wells167927B: Wash Buffer (20X), 1x25ml*167927C: Standard, 2x1vial167927D: Obestatin Pab, 2x1vial167927E: Assay Diluent A (For serum/plasma), 1x30ml167927F: Assay Diluent B (5X) (cell culture medium and other sampe types), 1x15ml*167927G: Obestatin (Biotin), 2x1vials167927H: Streptavidin (HRP) (1000X), 1x600ul*167927J: Positive Control, 1x1vial167927K: TMB, 1x12ml167927L: Stop Solution, 1x8mlStorage and Stability:Store 167927C, 167927G and 167927J at -70°C. Store other components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.