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中文名称: PDE1B Cell-Based Reporter BioAssay Kit

英文名字: PDE1B Cell-Based Reporter BioAssay Kit

供应商: USBiological

产品货号: 171960

产品报价: ￥1/500Tests

产品说明书

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: PDE1B plays a key role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE1B hydrolyzes both cAMP and cGMP to nucleoside 5′-monophosphate and prefers cGMP at low substrate levels. The PDE1B Cell-Based Reporter BioAssay Kit is designed for identification of PDE1B inhibitors in cultured cells. The kit includes a recombinant HEK293 cell line that stably expresses human PDE1B, atrial natriuretic peptide (ANP), mammalian expression constructs for ANP receptor A, CNGA2, and aequorin, and buffer for the assay. In the PDE1B reporter assay, PDE1B-HEK293 cells are transiently transfected with expression vectors for atrial natriuretic peptide (ANP) receptor A (ANPRA, a guanylate cyclase), cyclic nucleotide-gated cation channel CNGA2, and the photoprotein aequorin. When cells are activated with ANP, ANP induces the production of cGMP through ANPRA. cGMP can activate the CNGA2 channel and induces Ca2+ influx, resulting in aequorin luminescence. Therefore, the intracellular cGMP level is monitored via aequorin luminescence induced by Ca2+ influx through CNGA2, acting as the intracellular cGMP sensor. PDE1B can reduce the ANP-induced cGMP level in the cells, causing lowered aequorin luminescence. When PDE1B activity is inhibited (for example, by the PDE1 inhibitor BAY607550), then the ANP-induced cGMP level increases, resulting in calcium influx that stimulates luminescence signals. A schematic presentation of this assay is shown in figure 1.Components:PDE1B-HEK293 cell line, 1x1vialANPRA/aequorin Expression vector*, 1x20ug (80ng DNA/ul)CNGA2 Expression vector* 1x20ug (80ng DNA/ul)Atrial natriuretic peptide (ANP), 10um, 1x50ulCoelenterazine, 5mM, 1x50ulCa2+ free assay buffer, 1x100mlBSA (100X), 1x500ulCaCl2, 1M, 1x200ul*The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria. Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

产品特点: 针对PDE1B Cell-Based Reporter BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到PDE1B Cell-Based Reporter BioAssay Kit产品后将其置于-20°C/-70°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。