Bone Alkaline Phosphatase (BALP) BioAssay ELISA Kit (Rat) discontinued-Bone Alkaline Phosphatase (BALP) BioAssay ELISA Kit (Rat) discontinued代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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中文名称: Bone Alkaline Phosphatase (BALP) BioAssay ELISA Kit (Rat) discontinued

英文名字: Bone Alkaline Phosphatase (BALP) BioAssay ELISA Kit (Rat) discontinued

供应商: USBiological

产品货号: 183576

产品报价: ￥1/48Test ￥1/96Tests

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产品描述: The BioAssay ELISA Kit is a 1.5 hour solid-phase competitive ELISA designed for the quantitative determination of Bone Alkaline Phosphatase (BALP) in rat serum, plasma, cell culture supernatant, tissue homogenates and body fluids. This kit is for research use only, not for therapeutic or diagnostic applications.Assay Principle:Bone Alkaline Phosphatase (BALP) BioAssay ELISA Kit (Rat) utilizes the quantitative Competitive Enzyme Immunoassay technique, utilizing a monoclonal antibody and a conjugate to Bone Alkaline Phosphatase (BALP) (Rat). The assay sample and buffer are incubated together with the HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Target Protein concentration since Protein from samples and Protein-HRP conjugate compete for the anti-Protein antibody binding site. Since the number of sites is limited, as more sites are occupied by Protein from the sample, fewer sites are left to bind the Protein-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Target Protein concentration in each sample is interpolated from this standard curve.Detection Range:0.5-10ng/mlSensitivity:0.1ng/mlPrecision:Intra-Assay %CV: <10Inter-Assay %CV: <10Specificity:This assay has high sensitivity and excellent specificity for detection of Bone Alkaline Phosphatase (BALP). No significant crossreactivity or interference with analogs was observed. Note: Crossreactivity detection between BALP and all analogs has not been determined and crossreaction may exist in some cases.Kit Components:183576A: Microtiter Strips, 1x48 wells or 1x96 wells. Pre-coated with anti-BALP antibody.183576B: Enzyme Conjugate, 1x3ml or 1x6ml183576C: Standard, 0ng/ml, 1x1 vial183576D: Standard, 0.5ng/ml, 1x1 vial183576E: Standard, 1ng/ml, 1x1 vial183576F: Standard, 2.5ng/ml, 1x1 vial183576G: Standard, 5ng/ml, 1x1 vial183576H: Standard, 10ng/ml, 1x1 vial183576J: Substrate A, 1x6ml183576K: Substrate B, 1x6ml183576L: Stop Solution, 1x6ml183576M: Wash Solution, 100X, 1x10ml183576N: Balance Solution, 1x3mlNote: The BALANCE SOLUTION is used only when the sample is cell culture supernatants, body fluid and tissue homogenate; if the sample is serum or plasma, then the BALANCE SOLUTION is a superfluous reagentStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.netTech Support: tech@usbio.netCustomer Service: service@usbio.netTel: 781-639-5092 • 800-520-3011Fax: 978-594-8052

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其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。