The BioAssay ELISA Kit is a 1.5 hour solid-phase competitive ELISA designed for the quantitative determination of Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM1) in Mouse serum, plasma, cell lysates, cell culture supernatants, tissue homogenates and other body fluids. This kit for research use only, not for therapeutic or diagnostic applications.Detection Range: 250-5000pg/mlSensitivity: 1pg/mlPrecision:Intra-assay CV%: 5.3, 7.2Inter-assay CV%: 4.2, 5.4Specificity: This assay has high sensitivity and excellent specificity for detection of CEACAM1 (Mouse). No significant crossreactivity or interference with analogs was observed.Note: Crossreactivity detection between the CEACAM1 and all analogs has not been determined and crossreaction may exist in some cases.Kit Components:185170A: Microtiter Strips, 1x48 wells or 1x96 wells185170B: Enzyme Conjugate, 1x3ml or 1x6ml185170C: Standard, 0pg/ml, 1x1 vial185170D: Standard, 250pg/ml, 1x1 vial185170E: Standard, 500pg/ml, 1x1 vial185170F: Standard, 1000pg/ml, 1x1 vial185170G: Standard, 2500pg/ml, 1x1 vial185170H: Standard, 5000pg/ml, 1x1 vial185170J: Substrate A, 1x6ml185170K: Substrate B, 1x6ml185170L: Stop Solution, 1x6ml185170M: Wash Solution (100X), 1x10ml185170N: Balance Solution, 1x3mlNote:The BALANCE SOLUTION is used only when the sample is cell culture supernatants, body fluid and tissue homogenate; if the sample is serum or plasma, then the BALANCE SOLUTION is a superfluous reagent. Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Assay Principle:The Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM1) BioAssay ELISA kit employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate that is has been pre-coated with a monoclonal anti-CEACAM1 antibody. The assay samples and standards are incubated together with CEACAM1-HRP conjugate for one hour. A competition for limited antibody binding sites on the plate occurs between CEACAM1-HRP conjugate and CEACAM1 in the samples and standards. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to terminate the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CEACAM1 concentration in the sample or standard. A standard curve is plotted relating the intensity of the color (O.D.) to the concentrations of the standards. The CEACAM1 concentration in each sample is interpolated from this standard curve.Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.netTech Support: tech@usbio.netCustomer Service: service@usbio.netTel: 781-639-5092 • 800-520-3011Fax: 978-594-8052