Ghrelin BioAssay ELISA Kit (Rat) discontinued-Ghrelin BioAssay ELISA Kit (Rat) discontinued代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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中文名称: Ghrelin BioAssay ELISA Kit (Rat) discontinued

英文名字: Ghrelin BioAssay ELISA Kit (Rat) discontinued

供应商: USBiological

产品货号: 192886

产品报价: ￥1/48Tests ￥1/96Tests

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: The BioAssay ELISA Kit is a 1.5 hour solid-phase Sandwich ELISA designed for the quantitative determination of Ghrelin (Rat) in rat serum, plasma, cell lysates, cell culture supernatants, body fluid and tissue homogenates. This kit for research use only, not for therapeutic or diagnostic applications.Assay Principle:Ghrelin BioAssay ELISA Kit (Rat) utilizes the quantitative Sandwich Enzyme Immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Ghrelin (Rat). Standards or samples are then added to the microtiter plate wells and if the protein is present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of protein present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for the protein are added to each well to “sandwich” the protein immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain the target protein and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The protein concentration in each sample is interpolated from this standard curve.Detection Range:0.5-10ng/mlSensitivity:0.1ng/mlPrecision:Intra-Assay CV: 5.6, 6.8%Inter-Assay CV: 4.4, 6.1%Specificity:This assay has high sensitivity and excellent specificity for detection of Ghrelin. No significant crossreactivity or interference with analogs was observed. Note: Crossreactivity detection between Ghrelin and all analogs has not been determined and crossreaction may exist in some cases.Kit Components:192886A: Microtiter Strip, 1x48 wells, 1x96 wells. Pre-coated with anti-rat Ghrelin antibody.192886B: Enzyme Conjugate, 1x6ml for 48 Tests, 1x10ml for 96 Tests192886C: Standard, 0ng/ml, 1x1 vial192886D: Standard, 0.5ng/ml, 1x1 vial192886E: Standard, 1.0ng/ml, 1x1 vial192886F: Standard, 2.5ng/ml, 1x1 vial192886G: Standard, 5.0ng/ml, 1x1 vial192886H: Standard, 10ng/ml, 1x1 vial192886J: Substrate A, 1x6ml192886K: Substrate B, 1x6ml192886L: Stop Solution, 1x6ml192886M: Wash Solution, 100X, 1x10ml192886N: Balance Solution, 1x3mlStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

产品特点: 针对Ghrelin BioAssay ELISA Kit (Rat) discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到Ghrelin BioAssay ELISA Kit (Rat) discontinued产品后将其置于4°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。