Klotho (KL) BioAssay ELISA Kit (Rat) discontinued-Klotho (KL) BioAssay ELISA Kit (Rat) discontinued代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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中文名称: Klotho (KL) BioAssay ELISA Kit (Rat) discontinued

英文名字: Klotho (KL) BioAssay ELISA Kit (Rat) discontinued

供应商: USBiological

产品货号: 195659

产品报价: ￥1/48Tests ￥1/96Tests

产品说明书

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: The BioAssay ELISA Kit is a 1.5-hour solid-phase competitive ELISA designed for the quantitative determination of KL in rat serum, plasma, cell culture supernatants, tissue homogenates and other body fluids. This kit is intended for research use only and may not be used for therapeutic or diagnostic applications.Detection Range:100-2500pg/mlSensitivity:1.0pg/mlPrecision:Intra-Assay %CV: <10%Inter-Assay %CV: <10%Specificity:This assay has high sensitivity and excellent specificity for detection of KL (rat). No significant crossreactivity or interference with analogs was observed.Note: Crossreactivity detection between KL and all analogs has not been determined and crossreaction may exist in some cases.Assay Principle:Klotho (KL) BioAssay ELISA Kit (Rat) employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate that has been pre-coated with a monoclonal anti-KL antibody. The assay samples and standards are incubated together with KL-HRP conjugate for one hour. A competition for limited antibody binding sites on the plate occurs between KL-HRP conjugate and KL in the samples and standards. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to terminate the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KL concentration in the sample or standard. A standard curve is plotted relating the intensity of the color (O.D.) to the concentrations of the standards. The KL concentration in each sample is interpolated from this standard curve.Kit Components:195659A: Microtiter Strips, 1x48 wells or 1x96 wells195659B: Enzyme Conjugate, 1x3ml or 1x6ml 195659C: Standard, 0pg/ml, 1x1vial195659D: Standard, 100pg/ml, 1x1vial195659E: Standard, 250pg/ml, 1x1vial195659F: Standard, 500pg/ml, 1x1vial195659G: Standard, 1000pg/ml, 1x1vial195659H: Standard, 2500pg/ml, 1x1vial195659J: Substrate A, 1x6ml195659K: Substrate B, 1x6ml195659L: Stop Solution, 1x6ml195659M: Wash Solution, 100X, 1x10ml195659N: Balance Solution, 1x6mlNote: The BALANCE SOLUTION is used only when the sample is cell culture supernatants, body fluid and tissue homogenate; if the sample is serum or plasma, then the BALANCE SOLUTION is a superfluous reagent.Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.netTech Support: tech@usbio.netCustomer Service: service@usbio.netTel: 781-639-5092 • 800-520-3011Fax: 978-594-8052

产品特点: 针对Klotho (KL) BioAssay ELISA Kit (Rat) discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到Klotho (KL) BioAssay ELISA Kit (Rat) discontinued产品后将其置于4°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。