The L-Lactate Dehydrogenase B Chain (LDHB) BioAssay ELISA Kit (Rat) is a 1.5 hour solid-phase Competitive Enzyme Immunoassay designed for the quantitative determination of L-Lactate Dehydrogenase B Chain (LDHB) (Rat) in serum, plasma, cell culture supernatants, body fluid and tissue homogenates.Detection Range:25-500mU/mlSensitivity:1.0mU/mlPrecision:Intra-Assay CV: <10%Inter-Assay CV: <10%Specificity:This assay has high sensitivity and excellent specificity for detection of L-Lactate Dehydrogenase B Chain (LDHB) (Rat). No significant crossreactivity or interference with analogs was observed. Note: Crossreactivity detection between the LDHB and all analogs has not been determined and crossreaction may exist in some cases.Kit Components:195723A: Microtiter Plate, 48 wells, stripwell195723B: Enzyme Conjugate, 1x3ml195723C: Standard A, 0mU/ml, 1x1 vial195723D: Standard B, 25mU/ml, 1x1 vial195723E: Standard C, 50mU/ml, 1x1 vial195723F: Standard D, 100mU/ml, 1x1 vial195723G: Standard E, 250mU/ml, 1x1 vial195723H: Standard F, 500mU/ml, 1x1 vial195723J: Substrate A, 1x6ml195723K: Substrate B, 1x6ml195723L: Stop Solution, 1x6ml195723M: Wash Solution (100X), 1x10ml195723N: Balance Solution, 1x3ml Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Assay Principle:L-Lactate Dehydrogenase B Chain (LDHB) BioAssay ELISA Kit (Rat) utilizes the quantitative Competitive Enzyme Immunoassay technique, utilizing a monoclonal antibody and an conjugate to L-Lactate Dehydrogenase B Chain (LDHB) (Rat). The assay sample and buffer are incubated together with the HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LDHB concentration since LDHB from samples and LDHB-HRP conjugate compete for the anti-LDHB antibody binding site. Since the number of sites is limited, as more sites are occupied by LDHB from the sample, fewer sites are left to bind the LDHB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Target Protein concentration in each sample is interpolated from the standard curve.
产品特点
针对L-Lactate Dehydrogenase B Chain (LDHB) BioAssay ELISA Kit (Rat) discontinued该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到L-Lactate Dehydrogenase B Chain (LDHB) BioAssay ELISA Kit (Rat) discontinued产品后将其置于4°C保存。