Myosin (MYS) BioAssay ELISA Kit (Rat) discontinued-Myosin (MYS) BioAssay ELISA Kit (Rat) discontinued代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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中文名称: Myosin (MYS) BioAssay ELISA Kit (Rat) discontinued

英文名字: Myosin (MYS) BioAssay ELISA Kit (Rat) discontinued

供应商: USBiological

产品货号: 197380

产品报价: ￥1/48Tests ￥1/96Tests

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产品描述: Myosin (MYS) BioAssay ELISA Kit (Rat) is a 1.5 hour solid-phase Sandwich ELISA designed for the quantitative determination of Myosin (MYS) (Rat) in human serum, plasma, cell culture supernatants, body fluid and tissue homogenates. This kit is intended for research use only and may not be used for therapeutic or diagnostic applications.Detection Range:2.5-50g/mlSensitivity:0.1ng/mlPrecision:Intra-Assay %CV: <10Inter-Assay %CV: <10Specificity: This assay has high sensitivity and excellent specificity for detection of MYS (Rat). No significant crossreactivity or interference with analogs was observed. Note: Crossreactivity detection between the MYS and all analogs has not been determined and crossreaction may exist in some cases.Kit Components:197380A: Microtiter Strips, 1x48 wells or 1x96 wells197380B: Enzyme Conjugate, 1x6ml or 1x10ml197380C: Standard, 0ng/ml, 1x1 vial197380D: Standard, 2.5ng/ml, 1x1 vial197380E: Standard, 5ng/ml, 1x1 vial197380F: Standard, 10ng/ml, 1x1 vial197380G: Standard, 25ng/ml, 1x1 vial197380H: Standard, 50ng/ml, 1x1 vial197380J: Substrate A, 1x6ml197380K: Substrate B, 1x6ml197380L: Stop Solution, 1x6ml197380M: Wash Solution (100X), 1x10ml197380N: Balance Solution, 1x3mlNote:The BALANCE SOLUTION is used only when the sample is cell culture supernatants, body fluid and tissue homogenate; if the sample is serum or plasma, then the BALANCE SOLUTION is a superfluous reagent. Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Assay Principle:Myosin (MYS) BioAssay ELISA Kit (Rat) utilizes the quantitative Sandwich Enzyme Immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MYS (Rat). Standards or samples are then added to the microtiter plate wells and if the protein is present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of protein present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for the protein are added to each well to “sandwich” the protein immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain the target protein and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The protein concentration in each sample is interpolated from this standard curve.Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.netTech Support: tech@usbio.netCustomer Service: service@usbio.netTel: 781-639-5092 • 800-520-3011Fax: 978-594-8052

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保存建议: 建议收到Myosin (MYS) BioAssay ELISA Kit (Rat) discontinued产品后将其置于4°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。