【干冰】Anti-Hsp70 (MOUSE) Monoclonal Antibody - 200-301-A27-【干冰】Anti-Hsp70 (MOUSE) Monoclonal Antibody - 200-301-A27代理/参数/厂家-一抗-开云·官方站线上登录平台入口

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中文名称: 【干冰】Anti-Hsp70 (MOUSE) Monoclonal Antibody - 200-301-A27

英文名字: 【干冰】Anti-Hsp70 (MOUSE) Monoclonal Antibody - 200-301-A27

供应商: Rockland

产品货号: 200-301-A27

产品报价: ￥询价/200ug

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背景资料: Anti-Hsp 70 antibody is routinely used in cancer and signal transduction research. Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes, hsp70 genes exist as part of a multigene family. Hsp70s are found in most cellular compartments of eukaryotes, including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two-thirds of hsp70s are more conserved than the C-terminal one-third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins, preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization, and protein transport.

应用类型: Immunohistochemistry,IF Microscopy,Western Blot,

免疫原: Hsp70 monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide from the region of amino acid residues 436-503 of human Hsp70 protein.

来源宿主: Mouse

反应性: Bovine (Bos Taurus); Caenorhabditis elegans; Canine (Dog); Cavia porcellus (Guinea Pig); Cricetinae (Hamster); Cyprinidae (Carp); Drosophila melanogaster (Fruit fly); Gallus gallus (Chicken); H. sapiens (Human); Leporidae (Rabbit); Mus musculus (Mouse); Ovis aries (Sheep); Rattus (Rat); Simiiformes (Monkey); Sus scrofa domesticus (Pig, Porcine, Swine)

保存建议: Hsp70 antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

其他: Rockland成立于1962年，是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。