Esterase is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. It is active towards triacylglycerides containing short-chain fatty acids from C2 to C6, and 13-monoacylglycerols containing fatty acids from C2 to C12; and inactive on long-chain triacylglycerols and diacylglycerol. it hydrolyzes aromatic and alkyl esters and vitamin A acetate. The hydrolysis rate depends upon the amino acid promoiety and the esterification site of the prodrug. Aromatic promoieties are favored, highest rates are observed with phenylalanyl progdrugs, hydrolysis of valyl and isoleucyl prodrugs is less efficient. With floxuridine prodrugs, activity is higher on 5' monoesters than on 3' monoesters; while with gemcitabine prodrugs, activity is higher on 3' monoesters than on 5' monoesters.
应用类型
ELISA,Western Blot,
免疫原
Esterase [Porcine Liver]
来源宿主
Rabbit
反应性
Sus scrofa domesticus (Pig, Porcine, Swine)
保存建议
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.