The BioAssay ELISA Kit is a 1.5 hour solid-phase competitive ELISA designed for the quantitative determination of Orexin Receptor Type 1 (HCRTR1) (Rat). This kit for research use only, not for therapeutic or diagnostic applications.Detection Range:250-5000pg/mLSensitivity:1.0pg/mlIntra-Assay CV:<10% Inter-Assay CV:<10%Assay Principle:Orexin Receptor Type 1 (HCRTR1) BioAssay ELISA Kit (Rat) utilizes the quantitative Competitive Enzyme Immunoassay technique, utilizing a monoclonal antibody and an conjugate to Orexin Receptor Type 1 (HCRTR1) (Rat). The assay sample and buffer are incubated together with the HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Target Protein concentration since Protein from samples and Protein-HRP conjugate compete for the anti-Protein antibody binding site. Since the number of sites is limited, as more sites are occupied by Protein from the sample, fewer sites are left to bind the Protein-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Target Protein concentration in each sample is interpolated from this standard curve.Kit Components:200720A: Microtiter Strips, 1x48 wells or 1x96 wells. Pre-coated with anti-rat HCRTR1 antibody.200720B: Enzyme Conjugate, 1x3ml for 48Tests; 1x6ml for 96Tests.200720C: Standard, 0pg/ml, 1x1vial200720D: Standard, 250pg/ml, 1x1vial200720E: Standard, 500pg/ml, 1x1vial200720F: Standard, 1000pg/ml, 1x1vial200720G: Standard, 2500pg/ml, 1x1vial200720H: Standard, 5000pg/ml, 1x1vial200720J: Substrate A, 1x6ml200720K: Substrate B, 1x6ml200720L: Stop Solution, 1x6ml200720M: Wash Solution, 100X, 1x10ml200720N: Balance Solution, 1x3mlStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.netTech Support: tech@usbio.netCustomer Service: service@usbio.netTel: 781-639-5092 • 800-520-3011Fax: 978-594-8052
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