Tenascin C (TNC) BioAssay ELISA Kit (Rat) discontinued-Tenascin C (TNC) BioAssay ELISA Kit (Rat) discontinued代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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中文名称: Tenascin C (TNC) BioAssay ELISA Kit (Rat) discontinued

英文名字: Tenascin C (TNC) BioAssay ELISA Kit (Rat) discontinued

供应商: USBiological

产品货号: 204729

产品报价: ￥1/48Tests ￥1/96Tests

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产品描述: Tenascin C (TNC) BioAssay ELISA Kit (Rat) is a 1.5 hour solid-phase Sandwich ELISA designed for the quantitative determination of Tenascin C (TNC) (Rat) in serum, plasma, cell culture supernatants, body fluid and tissue homogenates.Detection Range:0-50ng/mlSensitivity:0.1ng/mlPrecision:Intra-Assay CV: <10%Inter-Assay CV: <10%Specificity:This assay has high sensitivity and excellent specificity for detection of Tenascin C (TNC) (Rat). No significant crossreactivity or interference with analogs was observed. Note: Crossreactivity detection between the TNC and all analogs has not been determined and crossreaction may exist in some cases.Kit Components:204729A: Microtiter Plate, 1x96 wells, stripwell204729B: Enzyme Conjugate, 1x10ml204729C: Standard A, 0ng/ml, 1x1 vial204729D: Standard B, 2.5ng/ml, 1x1 vial204729E: Standard C, 5.0ng/ml, 1x1 vial204729F: Standard D, 10ng/ml, 1x1 vial204729G: Standard E, 25ng/ml, 1x1 vial204729H: Standard F, 50ng/ml, 1x1 vial204729J: Substrate A, 1x6ml204729K: Substrate B, 1x6ml204729L: Stop Solution, 1x6ml204729M: Wash Solution (100X), 1x10ml204729N: Balance Solution, 1x3mlStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Assay Principle:Tenascin C (TNC) BioAssay ELISA Kit (Rat) utilizes the quantitative Sandwich Enzyme Immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Tenascin C (TNC) (Rat). Standards or samples are then added to the microtiter plate wells and if the protein is present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TNC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TNC are added to each well to “sandwich” the TNC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TNC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNC concentration in each sample is interpolated from this standard curve.

产品特点: 针对Tenascin C (TNC) BioAssay ELISA Kit (Rat) discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到Tenascin C (TNC) BioAssay ELISA Kit (Rat) discontinued产品后将其置于4°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。