Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor.SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages.Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells.Recombinant rat SCF is an 18.4kD polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.Source:Recombinant protein corresponding to rat SCF expressed in E. coli.Biological Activity:Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 for this effect is 20-40ng/ml.Endotoxin:<0.1ng/ug of protein (<1EU/ug)AA Sequence:MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVLPSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKLGKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIFNRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFMLPPVAProtein Content:Verified by UV Spectroscopy and/or SDS-PAGE gel.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with H2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.