7kb).Source:Recombinant protein corresponding to MMLV, expressed in E. coli strain.Applications:1. 1st strand cDNA synthesis. 2. Primer extension. 3. RT-PCR.Standard cDNA Synthesis Conditions: 50mM Tris-HCl, pH 8.3, 75mM KCl, 3mM MgCl2, 10mM DTT, 1.0mM each dATP, dGTP, dCTP, and dTTP, 0.2mg radom hexamer,1-5mg RNA, 200units M-MLV RT. The reaction volume was 20ml and the incubation was 45 min at 42oC.5X Reaction Buffer: 250mM Tris-HCl, pH 8.3, 375mM KCl, 15mM MgCl2, 50mM DTT.Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 1nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37oC, using poly(A)-oligo(dT)12-18 as the template-primer.Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.">
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