This rat EGF (Epidermal Growth Factor) BioAssay ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. Sample Type:Serum and plasma (heparin, EDTA). Cell culture supernatants, urine and tissue homogenates.Intended Use:For quantitative protein detection, establishing normal range etc.Sensitivity:<1pg/mlRange:7.8-500pg/mlSpecificity:Recognizes rat EGF. This ELISA kit shows no cross-reactivity with other relevant proteins.Test Principle:This assay employs a monoclonal antibody from mouse specific for EGF coated on a 96-well plate. Standards (E. Coli) and test samples are added to the wells and EGF present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for EGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat EGF captured onto the plate. Kit Components:Microplate coated with anti-rat monoclonal Ab against EGF, 1x96 wells Lyophilized recombinant rat EGF standard (10ng/vial), 2x vials Biotinylated anti-rat EGF antibody, 1x130ul Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent (Colorless), 1x10ml TMB stop solution, 1x10ml Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.