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中文名称: Fibronectin, BioAssay ELISA Kit (Rat)

英文名字: Fibronectin, BioAssay ELISA Kit (Rat)

供应商: USBiological

产品货号: 219770

产品报价: ￥1/100Tests

产品说明书

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: Fibronectin is a 430kD dimeric glycoprotein that exists in 2 forms - cellular and plasma fibronectin. Sample Type:Serum and plasma (heparin, EDTA, Citrate), cell culture supernatants Intended Use:For quantitative protein detection, establishing normal range.Sensitivity:<15pg/mlRange:156-10,000pg/mlSpecificity:Recognizes native and recombinant rat Fibronectin. This ELISA kit shows no cross-reactivity with other relevant proteins.Test Principle:This rat Fibronectin BioAssay ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for rat Fibronectin is coated on a 96-well plate. Standards and test samples are added to the wells and Fibronectin present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for Fibronectin is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat Fibronectin captured onto the plate.Kit Components:Microplate coated with anti-rat monoclonal Fibronectin antibody, 1x96 wells  Lyophilized native rat Fibronectin standard (10ng/vial), 2x vials  Biotinylated anti-rat Fibronectin antibody, 1x130ul  Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul  Sample diluent buffer, 1x30ml  Antibody diluent buffer, 1x12ml  ABC diluent buffer, 1x12ml  TMB color developing agent (Colorless), 1x10ml  TMB stop solution, 1x10ml Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

产品特点: 针对Fibronectin, BioAssay ELISA Kit (Rat)该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到Fibronectin, BioAssay ELISA Kit (Rat)产品后将其置于4°C/-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。