IL-1alpha, BioAssay ELISA Kit (Interleukin-1 alpha) (Rat)-IL-1alpha, BioAssay ELISA Kit (Interleukin-1 alpha) (Rat)代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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中文名称: IL-1alpha, BioAssay ELISA Kit (Interleukin-1 alpha) (Rat)

英文名字: IL-1alpha, BioAssay ELISA Kit (Interleukin-1 alpha) (Rat)

供应商: USBiological

产品货号: 219920

产品报价: ￥1/100Tests

产品说明书

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: The rat IL-1 alpha BioAssay ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 alpha.Sample Type:Serum, plasma and cell culture supernatantsIntended Use:For use in quantitative protein detection, establishing normal range and validation of antibody array results.Sensitivity:The minimum detectable dose of IL-1 alpha is typically <15pg/ml.Range:15-8000pg/mlSpecificity:Recognizes rat IL-1 alpha. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1b, IL-4, IL-6, IL-10, GM-CSF, IFN-g, Leptin, Lix, MCP-1, MIP-3a, b-NGF, TIMP-1, TNF-a.Test Principle:This BioAssay employs an antibody specific for IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.Kit Components:IL-1 alpha Coated Microplate (Item A), 1x96wells20X Wash Buffer Concentrate (Item B), 1x25mlStandards (Item C) (recombinant rat IL-1 alpha), 2x vialsAssay Diluent C (Item L) for Standard/Sample (serum/plasma/cell culture medium), 1x30ml Assay Diluent B (Item E) for detection antibody & HRP-streptavidin diluent, 5x conc., 1x15ml Detection Antibody IL-1 alpha (Item F), 2x vialHRP-Streptavidin Concentrate (Item G), 500x concentrated, 1x200ulTMB One-Step Substrate Reagent (Item H), 1x12mlStop Solution (Item I), 0.2M sulfuric acid, 1x8mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

产品特点: 针对IL-1alpha, BioAssay ELISA Kit (Interleukin-1 alpha) (Rat)该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到IL-1alpha, BioAssay ELISA Kit (Interleukin-1 alpha) (Rat)产品后将其置于-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。