MTS Cell Proliferation Colorimetric BioAssay Kit is a colorimetric method for sensitive quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the reduction of MTS tetrazolium compound by viable cells to generate a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes in metabolically active cells. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 490-500nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. This MTS assay is performed by adding the reagent directly into the cell culture media without the intermittent steps, which are required in the routine MTT assay. In addition, this high-throughput assay requires no washing or solubilization step and can be performed in 96-well microtiter plate.Applications:•Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients•Analysis of cytotoxic and cytostatic compounds such as anti-cancer drugs, toxic agents and other pharmaceuticals•Assessment of physiological mediators that inhibit cell growthSample Type:Adherent and suspension cellsKit Component:220081A: MTS Reagent (in electron coupling solution), 1x50mlStorage and Stability:Store other component at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.