The rat Progranulin BioAssay ELISA kit is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human progranulin in biological fluids. Sample Type:Serum and cell culture supernatantsSensitivity:The lowest level of progranulin that can be detected by this assay is 40pg/ml. Range:0.063-4ng/ml Specificity:Recognizes natural and recombinant rat Progranulin. It does not cross-react with human progranulin, human granulin C, human granulin F, rat RBP4, rat adiponectin, rat Nampt, rat ANGPTL4, rat lipocalin-2, rat resistin, mouse RBP4, mouse adiponectin, human RBP4, human adiponectin.Test Principle:A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of progranulin in the samples.Kit Components:Plate coated with rat Progranulin antibody, 1x96wells!0X Wash Buffer, 1x50mlDiluent 5X, 1x50mlDetection Antibody, 1x12mlDetector 100X (HRP Labeled Streptavidin), 1x150ulRat Progranulin Standard, lyophilized, 1x8ngRat Progranulin QC sample, lyophilized, 1x200ulTMB Substrate solution, 1x12mlPlate Sealers, 3x plastic filmStop Solution, 1x12mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.