Sample Type:Serum, plasma and urineIntended Use:For the quantitative determination of rat Microalbuminuria (MAU/ALB) concentrations in serum, plasma and urine. Sensitivity:0.01ug/mlRange:0.01-40ug/mlSpecificity:This assay has high sensitivity and excellent specificity for detection of MAU/ALB. No significant cross-reactivity or interference between MAU/ALB and analogues was observed.Precision:Intra-assay: CV% <8% Inter-assay: CV% <10% Test Principle:This BioAssay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for MAU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAU is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAU bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components:Assay plate, 1x96 wellsStandard (100X), 1x100ul Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x10ml HRP-avidin Diluent, 1x20ml Sample Diluent, 2x20ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.