Sample Type:SerumIntended Use:For the qualitative determination of rat anti-toxoplasmosis antibody (IgG) concentrations in serum. Specificity:This assay has high sensitivity and excellent specificity for detection of rat anti-toxoplasmosis antibody (IgG). No significant cross-reactivity or interference between rat anti-toxoplasmosis antibody (IgG) and analogues was observed.Test Principle:This BioAssay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-rat IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of rat anti-toxoplasmosis antibody (IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components:Coated assay plate, 1x96 wellsNegative Control, 1x0.5ml Positive Control, 1x0.5ml Sample Diluent, 2x20ml HRP-conjugate, 1x12ml Wash Buffer (20X), 1x30ml Substrate A, 1x7ml Substrate B, 1x7ml Stop Solution, 1x7ml Adhesive Strip (For 96 wells), 4x strips Instruction manual Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.