Procollagen I N-Terminal Propeptide (PINP), BioAssay ELISA Kit is a competitive inhibition immunoassay for in vitro quantitative measurement of PINP in rat serum, plasma, tissue homogenates and other biological fluidsDetection Range:2.47-200ng/mlSensitivity:0.92ng/mlIntra-Assay CV: <10%Inter-Assay CV: <12%Kit Components:*254324A: Microtiter Strips, 1x48 wells or 1x96 wells *254324B: Standard, 2x1 vial254324C: Standard Diluent, 1x10ml or 1x20ml*254324D: Detection Reagent A (green), 1x60ul or 1x120ul*254324E: Detection Reagent B(red), 1x60ul or 1x120ul254324F: Assay Diluent A, 1x6ml or 1x12ml254324G: Assay Diluent B, 1x6ml or 1x12ml254324H: TMB Substrate, 1x4.5ml or 1x9ml*254324J: Positive Control 65.7ng/ml, 1x1vial254324K: Stop Solution, 1x3ml or 1x6ml254324L: Wash Buffer, 30X, 1x10ml or 1x20mlPrecaution:The Stop Solution (254324K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *254324A, *254324B, *254324D, *254324E and *254324J at -20°C. After reconstitution of *254324J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.5. Aspirate and wash 5 times.6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
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保存建议
建议收到Procollagen I N-Terminal Propeptide (PINP), BioAssay ELISA Kit (Rat)产品后将其置于4°C/-20°C保存。