Vasoactive intestinal peptide also known as VIP is a peptide hormone containing 28 amino acid residues. VIP is neuropeptide that belongs to a glucagon/secretin superfamily, the ligand of class II G protein-coupled receptors. VIP is produced in many tissues of vertebrates including the gut, pancreas, and suprachiasmatic nuclei of the hypothalamus in the brain.Sample Type:Serum and plasmaIntended Use:The kit is a competitive enzyme immunoassay for the in vitro quantitative measurement of VIP in rat serum, plasma; and other biological fluids not being tested.Sensitivity:0.5pg/mlRange:2.4-10,000pg/mlSpecificity:This assay has high sensitivity and excellent specificity for detection of rat VIP.Precision:Intra CV<10%; Inter CV <15%Test Principle:The immunoplate in this kit is pre-coated with secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the VIP antibody whose Fab fragment will be competitively bound by both biotinylated VIP peptide and peptide standard or targeted peptide in samples. The biotinylated VIP peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes TMB substrate solution. The intensity of the yellow is directly proportional to the amount of biotinylated VIP peptide SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. A standard curve of known VIP concentration can be established accordingly. The unknown VIP concentration in samples can be determined by extrapolation to this standard curve.Kit Components:Assay plate (12x8 coated Microwells)-1Primary antibody-2x6uLStandard peptide-2x10uLBiotinylated peptide-2x10uLHRP-Streptavidin concentrate-1x60uLAssay diluent (5x concentrate)-1x15mLWash buffer (20x concentrate)-1x25mLTMB substrate-1x12mLStop solution-1x8mLAdhensive strip-2Instruction manual-1.Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.