Vasopressin, also known as arginine vasopressin (AVP), antidiuretic hormone (ADH), is aneurohypophysial hormone found in most mammals. It is derived from a preprohormone precursor that is synthesized in the hypothalamus and stored in vesicles at the posterior pituitary. Most of it is stored in the posterior pituitary to be released into the bloodstream. However, some AVP may also be released directly into the brain, and plays an important role in social behavior, sexual motivation and pair bonding, and maternal responses to stress.Sample Type:Serum and plasmaIntended Use:The kit is a competitive enzyme immunoassay for the in vitro quantitative measurement of Vasopressin in rat serum, plasma; and other biological fluids not being tested.Sensitivity:7pg/mlRange:2.4-10,000pg/mlSpecificity:This assay has high sensitivity and excellent specificity for detection of rat Vasopressin.Precision:Intra CV<10%; Inter CV <15%Test Principle:The immunoplate in this kit is pre-coated with secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the vasopressin antibody whose Fab fragment will be competitively bound by both biotinylated vasopressin peptide and peptide standard or targeted peptide in samples. The biotinylated vasopressin peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes TMB substrate solution. The intensity of the yellow is directly proportional to the amount of biotinylated vasopressin peptide SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. A standard curve of known Vasopressin concentration can be established accordingly. The unknown Vasopressin concentration in samples can be determined by extrapolation to this standard curve.Kit Components:Assay plate (12x8 coated Microwells)-1Primary antibody-2x6uLStandard peptide-2x10uLBiotinylated peptide-2x10uLHRP-Streptavidin concentrate-1x80uLAssay diluent (5x concentrate)-1x15mLWash buffer (20x concentrate)-1x25mLTMB substrate-1x12mLStop solution-1x8mLAdhensive strip-2Instruction manual-1.Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.