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中文名称: BAFF (B-Cell Activating Factor) BioAssay ELISA Kit (Human)

英文名字: BAFF (B-Cell Activating Factor) BioAssay ELISA Kit (Human)

供应商: USBiological

产品货号: 354179

产品报价: ￥1/96Tests

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: The BAFF (B-Cell Activating Factor) BioAssay ELISA Kit (Human) is a quantitative sandwich assay for the detection of BAFF in human serum, plasma, tissue homogenates and other biological fluids.Detection Range:62.5-4000pg/mlSensitivity:<37.5pg/mlPrecision: .Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to BAFF. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to BAFF. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BAFF , Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BAFF in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:*354179A: Microtiter Strips, 8x12 wells.*354179B: Standard, 2 vials354179C: Sample/Standard Dilution Buffer, 1x20ml354179D: Antibody-Biotin (Concentrated), 1x120ul354179E: Antibody Dilution Buffer, 1x10ml354179F: Streptavidin-HRP Conjugate (SABC), 1x120ul354179G: SABC Dilution Buffer, 1x10ml354179H: TMB Substrate, 1x10ml354179J: Stop Solution, 1x10ml354179K: Wash Buffer (25X), 1x30mlStorage and Stability:Store unopened *354179A at 4ºC; store at -20ºC once opened. Store unopened *354179B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Unused kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.

产品特点: The BAFF (B-Cell Activating Factor) BioAssay ELISA Kit (Human) is a quantitative sandwich assay for the detection of BAFF in human serum, plasma, tissue homogenates and other biological fluids.Detection Range:62.5-4000pg/mlSensitivity:<37.5pg/mlPrecision: .Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to BAFF. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to BAFF. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BAFF , Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BAFF in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:*354179A: Microtiter Strips, 8x12 wells.*354179B: Standard, 2 vials354179C: Sample/Standard Dilution Buffer, 1x20ml354179D: Antibody-Biotin (Concentrated), 1x120ul354179E: Antibody Dilution Buffer, 1x10ml354179F: Streptavidin-HRP Conjugate (SABC), 1x120ul354179G: SABC Dilution Buffer, 1x10ml354179H: TMB Substrate, 1x10ml354179J: Stop Solution, 1x10ml354179K: Wash Buffer (25X), 1x30mlStorage and Stability:Store unopened *354179A at 4ºC; store at -20ºC once opened. Store unopened *354179B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Unused kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.

保存建议: 建议收到BAFF (B-Cell Activating Factor) BioAssay ELISA Kit (Human)产品后将其置于4°C/-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。