Corticosterone (CORT) BioAssay ELISA Kit (Rat)-Corticosterone (CORT) BioAssay ELISA Kit (Rat)代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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中文名称: Corticosterone (CORT) BioAssay ELISA Kit (Rat)

英文名字: Corticosterone (CORT) BioAssay ELISA Kit (Rat)

供应商: USBiological

产品货号: 354930

产品报价: ￥1/96Tests

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: The Corticosterone (CORT) BioAssay ELISA Kit is a quantitative competitive assay for the detection of CORT in rat serum, plasma, tissue homogenates and other biological fluids.Detection Range:2.81-180ng/mlSensitivity:<1.688ng/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with CORT. CORT in the standard or sample competes with a fixed amount of plate-coated CORT for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of CORT in the samples is determined by comparing the absorbance of the samples to the standard curve.Kit Components:*354930A: Microtiter Strips, 1x96 wells (8x12 wells).*354930B: Standard, 2x1 vial354930C: Sample/Standard Dilution Buffer, 1x20ml354930D: Antibody-Biotin (Concentrated), 1x60ul354930E: Antibody Dilution Buffer, 1x10ml354930F: Streptavidin (HRP) (SABC,) 1x120ul354930G: SABC Dilution Buffer, 1x10ml354930H: TMB Substrate, 1x10ml354930J: Stop Solution, 1x10ml354930K: Wash Buffer, 25X, 1x30mlStorage and Stability:Store unopened *354930A at 4ºC; store at -20ºC once opened. Store unopened *354930 at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards and samples to wells!2. Add 50ul standard or sample to each well.3. Add 50ul Antibody (Biotin) working solution to each well and incubate for 45 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results

产品特点: The Corticosterone (CORT) BioAssay ELISA Kit is a quantitative competitive assay for the detection of CORT in rat serum, plasma, tissue homogenates and other biological fluids.Detection Range:2.81-180ng/mlSensitivity:<1.688ng/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with CORT. CORT in the standard or sample competes with a fixed amount of plate-coated CORT for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of CORT in the samples is determined by comparing the absorbance of the samples to the standard curve.Kit Components:*354930A: Microtiter Strips, 1x96 wells (8x12 wells).*354930B: Standard, 2x1 vial354930C: Sample/Standard Dilution Buffer, 1x20ml354930D: Antibody-Biotin (Concentrated), 1x60ul354930E: Antibody Dilution Buffer, 1x10ml354930F: Streptavidin (HRP) (SABC,) 1x120ul354930G: SABC Dilution Buffer, 1x10ml354930H: TMB Substrate, 1x10ml354930J: Stop Solution, 1x10ml354930K: Wash Buffer, 25X, 1x30mlStorage and Stability:Store unopened *354930A at 4ºC; store at -20ºC once opened. Store unopened *354930 at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards and samples to wells!2. Add 50ul standard or sample to each well.3. Add 50ul Antibody (Biotin) working solution to each well and incubate for 45 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results

保存建议: 建议收到Corticosterone (CORT) BioAssay ELISA Kit (Rat)产品后将其置于4°C/-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。