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中文名称: Hepc (Hepcidin) BioAssay ELISA Kit (Rat)

英文名字: Hepc (Hepcidin) BioAssay ELISA Kit (Rat)

供应商: USBiological

产品货号: 356254

产品报价: ￥1/96Tests

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产品描述: Hepc (Hepcidin) BioAssay ELISA Kit is for the quantitative detection of Hepc in rat serum, plasma, tissue homogenates and other biological fluids.Range:78.125-5000pg/mlSensitivity:<46.875pg/mlPrecision:Intra-assay CV: <8%Inter-assay CV: <10%Assay Principle:This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Hepc antibody was pre-coated onto 96-well plates. And the biotin conjugated Hepc antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrate was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Hepc amount of sample captured on plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Hepc can be calculated.Kit Components:*35654A: Microtiter Strips, 1x48 wells or 1x96 wells *35654B: Standard, 1x1vial or 2x1vial35654C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml35654D: Antibody (Biotin) (Concentrated), 1x60ul or 1x120ul35654E: Antibody Dilution Buffer, 1x5ml or 1x10ml35654F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul35654G: SABC Dilution Buffer, 1x5ml or 1x10ml35654H: TMB Substrate, 1x5ml or 1x10ml35654J: Stop Solution, 1x5ml or 1x10ml35654K: Wash Buffer, 25X, 1x15ml or 1x30mlStorage and Stability:Store unopened *35654A at 4ºC. Once opened, store at -20ºC. Store *35654B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well for 90 minutes at 37°C3. add 100ul Biotin antibody working solution to each well for 60 minutes at 37°C4. Aspirate and wash 3 times5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculation of results

产品特点: Hepc (Hepcidin) BioAssay ELISA Kit is for the quantitative detection of Hepc in rat serum, plasma, tissue homogenates and other biological fluids.Range:78.125-5000pg/mlSensitivity:<46.875pg/mlPrecision:Intra-assay CV: <8%Inter-assay CV: <10%Assay Principle:This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Hepc antibody was pre-coated onto 96-well plates. And the biotin conjugated Hepc antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrate was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Hepc amount of sample captured on plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Hepc can be calculated.Kit Components:*35654A: Microtiter Strips, 1x48 wells or 1x96 wells *35654B: Standard, 1x1vial or 2x1vial35654C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml35654D: Antibody (Biotin) (Concentrated), 1x60ul or 1x120ul35654E: Antibody Dilution Buffer, 1x5ml or 1x10ml35654F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul35654G: SABC Dilution Buffer, 1x5ml or 1x10ml35654H: TMB Substrate, 1x5ml or 1x10ml35654J: Stop Solution, 1x5ml or 1x10ml35654K: Wash Buffer, 25X, 1x15ml or 1x30mlStorage and Stability:Store unopened *35654A at 4ºC. Once opened, store at -20ºC. Store *35654B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well for 90 minutes at 37°C3. add 100ul Biotin antibody working solution to each well for 60 minutes at 37°C4. Aspirate and wash 3 times5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculation of results

保存建议: 建议收到Hepc (Hepcidin) BioAssay ELISA Kit (Rat)产品后将其置于4°C/-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。