Osteocalcin BioAssay ELISA Kit (Rat) (Bone Gla Protein, OC, BGP)-Osteocalcin BioAssay ELISA Kit (Rat) (Bone Gla Protein, OC, BGP)代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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中文名称: Osteocalcin BioAssay ELISA Kit (Rat) (Bone Gla Protein, OC, BGP)

英文名字: Osteocalcin BioAssay ELISA Kit (Rat) (Bone Gla Protein, OC, BGP)

供应商: USBiological

产品货号: 357827

产品报价: ￥1/96Tests

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产品描述: The Osteocalcin BioAssay ELISA Kit is a quantitative sandwich assay for the detection of Osteocalcin in rat serum, plasma, tissue homogenates and other biological fluids.Detection Range:15.625-1000pg/mlSensitivity:<9.375pg/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to Osteocalcin. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteocalcin, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osteocalcin in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:*357827A: Microtiter Strips, 1x96 wells (8x12 wells)*357827B: Standard, 2x1 vial357827C: Sample/Standard Dilution Buffer, 1x20ml357827D: Antibody-Biotin (Concentrated), 1x120ul357827E: Antibody Dilution Buffer, 1x10ml357827F: Streptavidin-HRP Conjugate (SABC), 1x120ul357827G: SABC Dilution Buffer, 1x10ml357827H: TMB Substrate, 1x10ml357827J: Stop Solution, 1x10ml357827K: Wash Buffer, 25X, 1x30mlStorage and Stability:Store unopened *357827A at 4ºC; store at -20ºC once opened. Store unopened *357827B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells.2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times.3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.

产品特点: The Osteocalcin BioAssay ELISA Kit is a quantitative sandwich assay for the detection of Osteocalcin in rat serum, plasma, tissue homogenates and other biological fluids.Detection Range:15.625-1000pg/mlSensitivity:<9.375pg/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to Osteocalcin. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteocalcin, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osteocalcin in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:*357827A: Microtiter Strips, 1x96 wells (8x12 wells)*357827B: Standard, 2x1 vial357827C: Sample/Standard Dilution Buffer, 1x20ml357827D: Antibody-Biotin (Concentrated), 1x120ul357827E: Antibody Dilution Buffer, 1x10ml357827F: Streptavidin-HRP Conjugate (SABC), 1x120ul357827G: SABC Dilution Buffer, 1x10ml357827H: TMB Substrate, 1x10ml357827J: Stop Solution, 1x10ml357827K: Wash Buffer, 25X, 1x30mlStorage and Stability:Store unopened *357827A at 4ºC; store at -20ºC once opened. Store unopened *357827B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells.2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times.3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.

保存建议: 建议收到Osteocalcin BioAssay ELISA Kit (Rat) (Bone Gla Protein, OC, BGP) 产品后将其置于4°C/-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。