Phospho-JNK/SAPK Antibody,c-Jun kinase,JNK,JNK 1,JAK1A,MAPK8,MAPK 8,SAPK 1,SAPK1,Kinase JNK1,Stress activated protein kinase
供应商
Biovision
产品货号
3589-100
产品报价
¥已停产
产品说明书
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产品新闻
背景资料
The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines (such as TNF- and IL-1),UV light,inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1 (also known as MKK4),which in turn activates SAPK (also known as JNK). JNK binds tightly to the N-terminal region of c-Jun and ATF-2,and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71.
应用类型
Western blotting (1-2 ug/ml), immunoprecipitation (10-20 ug/ml), immunocytochemistry (10-20 ug/ml), and Immunohistochemistry (20-30 ug/ml). However, the optimal conditions should be determined individually. The antibody detects the p46 and p54 JNK/SAPK dually phosphorylated at Thr183 and Tyr185.
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK