TGF-beta1 BioAssay ELISA Kit (Human) (Transforming growth factor beta-1，TGF-beta-1，TGFB1，TGFB，TGF beta1，TGFbeta1)-TGF-beta1 BioAssay ELISA Kit(Human)(Transforming growth factor beta-1，TGF-beta-1，TGFB1，TGFB，TGF beta1，TGFbeta1)代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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中文名称: TGF-beta1 BioAssay ELISA Kit (Human) (Transforming growth factor beta-1，TGF-beta-1，TGFB1，TGFB，TGF beta1，TGFbeta1)

英文名字: TGF-beta1 BioAssay ELISA Kit(Human)(Transforming growth factor beta-1，TGF-beta-1，TGFB1，TGFB，TGF beta1，TGFbeta1)

供应商: USBiological

产品货号: 361302

产品报价: ￥1/48Tests

产品说明书

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: Transforming growth factor （TGF），a ‘factor’ that promoted the transformation of cultured fibroblasts into a tumor-like phenotype，was subsequently found to be more of a tumor suppressor than tumor promoter and to be a mixture of two proteins，TGF-alpha and TGF-beta. Human TGF-beta1 is a 25kD，disulfide-linked，non-glycosylated homodimer. There is nearly 100% sequence conservation across mammalian species. Biological activity requires release of TGF-beta1 from the latent complex. This can be done in vitro by disruption of LAP （e.g. acidification）. There are，however，three fundamental activities： TGF-beta1 modulates cell proliferation，generally as a supressor; TGF-beta1 enhances the deposition of extracellular matrix through promotion of synthesis and inhibition of degradation; TGF-beta1 is immunosuppressive through a variety of mechanisms. The specific action of TGF-beta on a particular cell depends on the exact circumstances of that cell’s environment.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human TGF-beta1 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human TGF-beta1 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：7pg/mlRange：31.25-2000pg/mlSpecificity：Recognizes human TGF-beta1. There was no detectable cross reactivity with any of the tested proteins following IL-1,2,3,4,5,6，8,10,12,TNF,VEGF,ICAM and IFN.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h TGF-beta1 monoclonal coating antibody is adsorbed onto microwells. TGF-beta1 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h TGF-beta1 antibody is added and binds to TGF-beta1 captured by the first antibody. Following incubation unbound Biotinylated anti-h TGF-beta1 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h TGF-beta1 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of TGF-beta1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TGF-beta1 standard dilutions and TGF-beta1 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x6Standard： 1xVialBiotinylated antibody： 1xVialStreptavidin-HRP： 1xVialAssay Buffer （25ml/Vial）： 1xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x6mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 2xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.

产品特点: 针对TGF-beta1 BioAssay ELISA Kit (Human) (Transforming growth factor beta-1，TGF-beta-1，TGFB1，TGFB，TGF beta1，TGFbeta1)该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到TGF-beta1 BioAssay ELISA Kit （Human）（Transforming growth factor beta-1，TGF-beta-1，TGFB1，TGFB，TGF beta1，TGFbeta1）产品后将其置于4°C至-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。